mab35D7.1 staining pattern

mab35D7.1 exclusively stains embryonic chordotonal organs

Beate Lichte, Thilo Schneider, and Karl-Friedrich Fischbach
Institut für Biologie III, Schänzlestr.1, 79104 Freiburg i. Brsg.

We recently raised murine monoclonal antibodies against the extracellular part of the IrreC-rst protein. As a by-product of this immunization we got a mouse monoclonal antibody (mab35D7.1) which recognizes exclusively all chordotonal organs of the Drosophila embryo. The staining is very strong without any background.

Labelling of chordotonal organs with monoclonal antibody 35D7.1 is visible for the first time in embryonic stage 13. In later stages all embryonic chordotonal organs are stained ( overviews: Fig.1, enlargements Fig.2 and Fig.3). Postembryonic chordotonal organs are not labelled. The Vectastain ABC-kit was used for detection of bound antibodies.

mab35D7.1 does not recognize the IrreC-rst fusionprotein used for immunization (in ELISAs), nor does it recognize the protein in wild type or in transformants overexpressing it. Staining of embryonic chordotonal organs is unaffected by null mutations in irreC-rst. None of our antibodies recognizing the IrreC-rst protein in wild type and transformants does label chordotonal organs. We conclude that the 35D7.1 antigen is not found on IrreC-rst.

Mab35D7.1 might be useful as a specific marker for embryonic chordotonal organs.

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