To: dros@net.bio.net
From: matthewk@fly.bio.indiana.edu ("Kathy Matthews")
Subject: Meister pattern data for BDGP stocks at Bloomington
Date: 5 Dec 1995 17:32:43 -0800
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Marie Meister (CNRS - IBMC, 15, rue Rene Descartes, 67084-Strasbourg, France, meister@astorg.u-strasbg.fr) has made available a large set of late third instar (wandering stage) lacZ staining pattern data for Berkeley Drosophila Genome Project enhancer-trap P insertion lines held at the Bloomington Stock Center. The data have been incorporated into the Bloomington stock list and are available for searching on FlyBase.
The following body-parts controlled vocabulary was used to enter these data into the stock list. Use one or more keywords in a FlyBase search of the stock center stock lists to see the relevant pattern and stock information. To see only lines with Meister data include 'and Meister' in your search string, e.g., 'trachea and Meister' (quotation marks are for clarity - don't use them in your search string).
anterior gut
brain
cuticle
epidermis
esophagus
eye-antennal disc
fat body
foregut imaginal ring
gastric caecum
gonad
hindgut
hindgut imaginal ring
imaginal disc
Malpighian tubule
midgut
midgut imaginal island
oenocyte
prothoracic gland
proventriculus
ring gland
somatic muscle
trachea

The following information is excerpted from Marie Meister's letter of transmittal to the Bloomington Stock Center:

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The staining has been done without fixation by direct dissection into a drop of X-Gal solution (0.2% X-gal, 3.5 mM K[[4]]Fe(CN)[[6]], 3.5 mM K[[3]]Fe(CN)[[6]], 1 mM MgCl[[2]], 150 mM NaCl, 10 mM Na[[2]]HPO[[4]], 10 mM NaH[[2]]PO[[4]] and 30% Ficoll). The omission of the fixation step allows a more rapid screen, but it may result in some inaccuracy in the brain area staining (the distinction between ring gland, imaginal discs and brain is somewhat difficult when the staining is strong and leaking). The indications concerning the gut staining, especially in the case of weak colorations, may be misleading because there are variable levels and patterns of endogenous galactosidase activities in these tissues, depending on the genetic background. The results are given in levels of staining intensity:

++ strong staining
+ intermediate
(+) weak
((+)) very weak

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Many thanks to Marie for making these data available.

-- Kathy

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Kathy Matthews Bloomington Drosophila Stock Center
Department of Biology MATTHEWK@FLY.BIO.INDIANA.EDU
Indiana University MATTHEWK@INDIANA.EDU
Bloomington, IN 47405-6801 812-855-5782